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Adenosine monophosphateactivated protein kinase in

Mar 08, 2016 Diabetic nephropathy (DN) is the leading cause of end-stage renal disease, and its pathogenesis is complex and has not yet been fully elucidated. Gowans G.J., Hawley S.A., Ross F.A., Hardie D.G. AMP is a true physiological regulator of AMP-activated protein kinase by both allosteric activation and enhancing net phosphorylation. Cell Metab.

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AMPKSensing Energy while Talking to Other Signaling

The AMP-activated protein kinase (AMPK) is a sensor of cellular energy and nutrient status, expressed almost universally in eukaryotes as heterotrimeric complexes comprising catalytic () and regulatory ( and ) subunits. Along with the mechanistic target of rapamycin complex-1 (mTORC1), AMPK may have been one of the earliest signaling pathways to have arisen during eukaryotic evolution.

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The Lysosomal vATPaseRagulator Complex Is a Common

AMPK and mTOR regulate cellular balance between catabolism and anabolism. Zhang et al. show that the endosomal v-ATPase-Ragulator complex, required for mTORC1 activation when nutrients are abundant, is also essential in LKB1-mediated AMPK activation in response to energy stress, thus acting as a dual energy sensor.

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Oxidative stress activates AMPK in cultured cells

Sep 17, 2014 Oxidative stress activates AMPK in cultured cells primarily by increasing cellular AMP and/or ADP. Gowans G.J., Hawley S.A., Ross F.A., Hardie D.G. AMP is a true physiological regulator of AMP-activated protein kinase by both allosteric activation and enhancing net phosphorylation.

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A Highly Sensitive NonRadioactive Activity Assay for AMP

While many methods exist to quantitatively determine protein kinase activities, 32P-based radioactive assays remain the workhorse of many laboratories due to their high sensitivity, high signal to noise ratio, lack of interference by fluorescent and light-absorbing small molecules, and easy quantitation. Here, we demonstrate that the interaction between the yeast Rad53 Forkhead-associated (FHA.

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